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nc 009783 1 nc 009784 1  (ATCC)
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ATCC nc 009783 1 nc 009784 1
Nc 009783 1 Nc 009784 1, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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calpastatin  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology calpastatin
Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and <t>calpastatin</t> (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells
Calpastatin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti enos  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology mouse anti enos
Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and <t>calpastatin</t> (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells
Mouse Anti Enos, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chr1 nc 009783 1  (ATCC)
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ATCC chr1 nc 009783 1
Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and <t>calpastatin</t> (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells
Chr1 Nc 009783 1, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna against tpx2  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology sirna against tpx2
Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and <t>calpastatin</t> (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells
Sirna Against Tpx2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tpx2 sirna  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology tpx2 sirna
Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and <t>calpastatin</t> (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells
Tpx2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cp000789  (ATCC)
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ATCC cp000789
Genomic features of the vibrios genomes.
Cp000789, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and calpastatin (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells

Journal: Cancer Cell International

Article Title: Changes in calpain-2 expression during glioblastoma progression predisposes tumor cells to temozolomide resistance by minimizing DNA damage and p53-dependent apoptosis

doi: 10.1186/s12935-023-02889-8

Figure Lengend Snippet: Temozolomide (TMZ) sensitivity of primary and established GBM cell lines. Error bars indicate the standard deviation. a Cell viability (MTT reduction assay; ANOVA and Tukey’s test) of primary, patient-derived GBM cells after 5-day exposure to TMZ ± calpain inhibition by PD150606 (PD, f.c. 50 µM). b Western blot analysis of five selected GBM cell lines for their CAPN1, CAPN2, and calpastatin (CAST) expression. GAPDH was used as loading control. c Cell viability (CellTiter Glo assay; ANOVA and Tukey’s test) of established GBM cell lines (n = 3) after 5-day TMZ ± 50 µM PD administration. d Cell death rate in U251N cells after 5-day TMZ (f.c. 70 µM) and PD (f.c. 50 µM) treatment. Cell death rate was counted as the number of propidium iodide (PI) positive cells relative to the total number of cell nuclei stained with Hoechst (n = 3, ANOVA and Tukey’s test). e Exemplary fluorescent images showing Hoechst stained nuclei (blue) and PI stained dead cells (red) after the indicated treatment. The numbers below the images indicate the percentage of PI positive nuclei in these images. f Calpain activity is increased in U251N cells upon 5-day TMZ administration (n = 3). Calpain activity (fluorometric intensity (Ex/EM 400/505 nm)) is calculated relative to the DMSO treated control cells

Article Snippet: Antibodies directed against calpain-1 (ab39170), calpain-2 (ab39165), GAPDH (ab8245) and tubulin beta III (ab7751) were obtained from Abcam; Calpastatin from Santa Cruz (sc376547), cleaved caspase-3 (Asp175) (5A1E) (#9664) and phospho-histone H2A.X (Ser139) (#2577) from Cell Signaling Technology, TP53 (21,891–1-AP) from Proteintech, and 53BP1 clone BP13 (MAB3802) from Sigma-Aldrich.

Techniques: Standard Deviation, MTT Reduction Assay, Derivative Assay, Inhibition, Western Blot, Expressing, Control, Glo Assay, Staining, Activity Assay

Genomic features of the vibrios genomes.

Journal: BMC Evolutionary Biology

Article Title: Genomic taxonomy of vibrios

doi: 10.1186/1471-2148-9-258

Figure Lengend Snippet: Genomic features of the vibrios genomes.

Article Snippet: Vibrio harveyi ATCC BAA-1116 , CP000789 , 3765351 , 45 , , 85 , 53.

Techniques: